Journal: iScience

Article Title: Engineering of thick human functional myocardium via static stretching and electrical stimulation

doi: 10.1016/j.isci.2022.103824

Figure Lengend Snippet: hCMPs were matured via static stretching with or without electrical stimulation hCMPs were assembled in three layers over a three-day period (one layer per day) and then cultured for one week before maturation. (A) hCMP thicknesses were measured before maturation. (B and C) One week after fabrication, hCMPs were cultured under standard conditions (i.e., without stretching or electrical stimulation), (B) while stretched between two lines of needles, or (C) with stretching and electrical stimulation. Electrical stimulation was applied in 2-ms pulses at 2 Hz and either 15 V or 22 V

Article Snippet: Here we utilized passive mechanical stimulation along with chronic biphasic electrical stimulation (C-Pace, IonOptix) ( ; ; ) over a 10 day period, half of the time utilized by to enhance the maturation of the hiPSC-CMs as well as the tissue overall.

Techniques: Cell Culture

Journal: iScience

Article Title: Engineering of thick human functional myocardium via static stretching and electrical stimulation

doi: 10.1016/j.isci.2022.103824

Figure Lengend Snippet: Static stretching with electrical stimulation was associated with more mature patterns of contractile protein expression After 10 days of culture under standard conditions (i.e., in the absence of stretching or electrical stimulation; Control), with stretching alone (Stretched), or with stretching and either 15 V (Stretched+15V) or 22 V (Stretched+22V) electrical stimulation, contractile proteins were visualized in whole-mounted hCMPs via immunofluorescent staining for the expression of cTnT and α-actinin; nuclei were counterstained with DAPI

Article Snippet: Here we utilized passive mechanical stimulation along with chronic biphasic electrical stimulation (C-Pace, IonOptix) ( ; ; ) over a 10 day period, half of the time utilized by to enhance the maturation of the hiPSC-CMs as well as the tissue overall.

Techniques: Expressing, Control, Staining

Journal: iScience

Article Title: Engineering of thick human functional myocardium via static stretching and electrical stimulation

doi: 10.1016/j.isci.2022.103824

Figure Lengend Snippet: Static stretching with 22-V electrical stimulation was associated with isoform switching of genes related to structural maturity The relative mRNA expression of maturation genes and their isoform ratios of (A) Myosin Heavy Chain Alpha and Beta, (B) Myosin Light Chain Atrial and Ventricular, and (C) Troponin I one and three were evaluated in Control, Stretched, and Stretched+22V hCMPs via qPCR; results were normalized to the abundance of GAPDH mRNA in each sample (∗p < 0.05, ∗∗p < 0.01; n = 3 per group).

Article Snippet: Here we utilized passive mechanical stimulation along with chronic biphasic electrical stimulation (C-Pace, IonOptix) ( ; ; ) over a 10 day period, half of the time utilized by to enhance the maturation of the hiPSC-CMs as well as the tissue overall.

Techniques: Expressing, Control

Journal: iScience

Article Title: Engineering of thick human functional myocardium via static stretching and electrical stimulation

doi: 10.1016/j.isci.2022.103824

Figure Lengend Snippet: Static stretching with 22-V electrical stimulation increased protein expression of key cardiac maturation components The abundance of protein for key structural and functional components were evaluated in Control and Stretched+22V hCMPs via western blotting; results were normalized to the abundance of Beta Actin protein in each sample (∗p < 0.05; n = 3 per group).

Article Snippet: Here we utilized passive mechanical stimulation along with chronic biphasic electrical stimulation (C-Pace, IonOptix) ( ; ; ) over a 10 day period, half of the time utilized by to enhance the maturation of the hiPSC-CMs as well as the tissue overall.

Techniques: Expressing, Functional Assay, Control, Western Blot

Journal: iScience

Article Title: Engineering of thick human functional myocardium via static stretching and electrical stimulation

doi: 10.1016/j.isci.2022.103824

Figure Lengend Snippet: Static stretching with 22-V electrical stimulation was associated with more mature levels of CM gene expression The abundance of mRNA for genes that contribute to the (A) calcium handling, (B) metabolic activity, and (C) structural function of CMs were evaluated in Control, Stretched, and Stretched+22V hCMPs via qPCR; results were normalized to the abundance of GAPDH mRNA in each sample (∗p < 0.05; n = 3 per group).

Article Snippet: Here we utilized passive mechanical stimulation along with chronic biphasic electrical stimulation (C-Pace, IonOptix) ( ; ; ) over a 10 day period, half of the time utilized by to enhance the maturation of the hiPSC-CMs as well as the tissue overall.

Techniques: Gene Expression, Activity Assay, Control

Journal: iScience

Article Title: Engineering of thick human functional myocardium via static stretching and electrical stimulation

doi: 10.1016/j.isci.2022.103824

Figure Lengend Snippet: Static stretching with electrical stimulation was associated with structural maturation of CMs (A and B) hCMP sections were imaged via TEM and analyzed qualitatively for functional components of the cardiomyocyte sarcomere (ECM: extracellular matrix, GJ: gap junction, MC: mitochondrion, N: nucleus, ZL: Z-line), and (B) sarcomere lengths were measured for each group (∗p < 0.05, ∗∗∗p < 0.005, ∗∗∗∗p < 0.0001; images taken from at least three hCMP per group with n = 25 separate fields-of-view per group)

Article Snippet: Here we utilized passive mechanical stimulation along with chronic biphasic electrical stimulation (C-Pace, IonOptix) ( ; ; ) over a 10 day period, half of the time utilized by to enhance the maturation of the hiPSC-CMs as well as the tissue overall.

Techniques: Functional Assay